Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability

Autor: Santi Devi Upadhaya, Jae Yong Song, Min Ah Park, Ja Kyeom Seo, Liu Yang, Chan Ho Lee, Kyung J. Cho, Jong K. Ha
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: Asian-Australasian Journal of Animal Sciences, Vol 25, Iss 1, Pp 114-121 (2012)
Druh dokumentu: article
ISSN: 1011-2367
1976-5517
DOI: 10.5713/ajas.2011.11104
Popis: The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at 39°C. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid ‘corn’ substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications.
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