Hypoosmotic swelling test in alpaca (Vicugna pacos) spermatozoa recovered the vas deferens
| Autor: | Quispe HA, Ciprián R, Ordoñez C, Ampuero E, Cucho H |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | SPERMOVA, Vol 5, Iss 1, Pp 10-14 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2223-9375 2308-4928 |
| DOI: | 10.18548/aspe/0002.2 |
| Popis: | The study objective was to evaluate the endosmotic reaction in 5 hypoosmotic solutions 100, 125, 150, 175 and 200 mOsm/l (HOS test) incubated 5 and 15 minutes using alpaca sperm recovered the vas deferens. We used 5 male alpacas with the vas deferens surgically diverted, recovering sperm 10 times for each animal; the microscopic characteristics were evaluated with ISAS®. Information’s hipoosmotic test was analyzed with a factorial arrangement in a random one-way model, the correlation between endosmosis and percentages of motility and vitality was analyzed with Spearman correlation. There were no significant differences (p>0,05) in incubation times; highly significant differences (p 0,05)was found to endosmosis with percentages of sperm motility and vitality; in conclusion, a 125 mOsm/l solution with 5 minutes of incubation is sufficient to perform the hipoosmotic test in alpaca spermatozoa recovered the vas deferens. |
| Databáze: | Directory of Open Access Journals |
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