Proteomics analysis reveals heat shock proteins involved in caprine parainfluenza virus type 3 infection

Autor: Chunyan Zhong, Jizong Li, Li Mao, Maojun Liu, Xing Zhu, Wenliang Li, Min Sun, Xinqin Ji, Fang Xiao, Leilei Yang, Wenwen Zhang, Zheng Liao
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: BMC Veterinary Research, Vol 15, Iss 1, Pp 1-14 (2019)
Druh dokumentu: article
ISSN: 1746-6148
07161859
DOI: 10.1186/s12917-019-1897-6
Popis: Abstract Background Caprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis. Results Quantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value 1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data. Conclusion To our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje