Molecular detection of Babesia ovis and blood parameters' investigation reveal haematological and biochemical alterations in babesiosis infected Lohi sheep in Multan, Pakistan
| Autor: | Muhammad Sajid, Syed Atif Hasan Naqvi, Muhammad Riaz, Ummad Ud Din Umar, Nasreen Nasreen, Adil Khan, Mourad Ben Said |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Open Veterinary Journal, Vol 13, Iss 11, Pp 1400-1408 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2226-4485 2218-6050 |
| DOI: | 10.5455/OVJ.2023.v13.i11.2 |
| Popis: | Background: Babesia infections in sheep can cause a wide range of clinical and laboratory presentations. Changes in Blood parameters are a meaningful manifestation of physiological and pathological changes in an organism. Aim: Therefore, the present study was conducted to analyze and compare haematological and biochemical parameters between blood profiles of Lohi sheep naturally infected and un-infected with Babesia ovis, the main causative agent of ovine babesiosis. Methods: Initially, blood and serum samples from 67 Lohi sheep were collected, DNA was extracted and babesial infection was detected through PCR. Overall infection rate of B. ovis was 37% (25/67). Sixteen infected (experiment group) and sixteen uninfected (control group) sheep that were apparently healthy with no history of previous treatment for Babesiosis, were selected for haemato-biochemical analysis. Blood samples were analyzed through an automatic CBC analyzer, while serum collected from gel-vacutainers was analyzed for blood urea, blood urea nitrogen (BUN), creatinine and total bilirubin. Each parameter was compared between infected and un-infected animals using a paired t-test in Minitab Express™ software for statistical analyses. Results: Erythron comparison showed a highly significant (P [Open Vet J 2023; 13(11.000): 1400-1408] |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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