Effect of Sodium on Methanogens in a Two-Stage Anaerobic System

Autor: Keke Xiao, Yogananda Maspolim, Yan Zhou, Chenghong Guo, Wun Jern Ng
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Applied Sciences, Vol 12, Iss 3, p 956 (2022)
Druh dokumentu: article
ISSN: 2076-3417
DOI: 10.3390/app12030956
Popis: This study evaluated the effects of sodium on anaerobic biomass from the second-stage reactor of a two-stage anaerobic digester. The results indicated that methanogens showed a relatively high sodium tolerance of 2.4 g Na+ L−1. Microbial community analysis showed that viable Methanomicrobiales was the most abundant population by a combined propidium monoazide cross-linking quantitative polymerase chain reaction technique. There was a population shift towards higher abundance of Thermotoga (0.02%), Clostridium (2.50%) and Methanoculleus (13.80%). Biomass activity in relation to increased sodium concentrations was investigated with the adenosine triphosphate test coupled with extracellular polymeric substances measurement. The results showed biomass activity decreased from 33 to 16 µg g−1 volatile suspended solids as sodium concentrations increased from 1.3 to 9.1 g Na+ L−1. Higher EPS production, particularly a greater predominance of carbohydrates, was stimulated by higher sodium concentrations. This study provides insights into the superiority of sodium tolerance of two-stage anaerobic digester in compared with a single-stage anaerobic system.
Databáze: Directory of Open Access Journals