Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

Autor: Litwińczuk Wojciech, Okołotkiewicz Ewa, Matyaszek Iwona
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Folia Horticulturae, Vol 21, Iss 2, Pp 43-52 (2009)
Druh dokumentu: article
ISSN: 2083-5965
DOI: 10.2478/fhort-2013-0137
Popis: In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and ‘Elsanta’ clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm-3), IBA (0.1 mg dm-3), glucose (40.0 g dm-3) and GA3 (0.1 - control, 1.0, 2.0, 5.0, 10.0 mg dm-3). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA3 applied at a concentration of 1.0-2.0 mg dm-3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm-3) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners, which could also reduce the risk of domination of in vitro cultures with adventitious shoots, might be elaborated
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