Characterization of d-xylose reductase, XyrB, from Aspergillus niger
| Autor: | Agata Terebieniec, Tania Chroumpi, Adiphol Dilokpimol, Maria Victoria Aguilar-Pontes, Miia R. Mäkelä, Ronald P. de Vries |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biotechnology Reports, Vol 30, Iss , Pp e00610- (2021) |
| Druh dokumentu: | article |
| ISSN: | 2215-017X 65173538 |
| DOI: | 10.1016/j.btre.2021.e00610 |
| Popis: | d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP.Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21). |
| Databáze: | Directory of Open Access Journals |
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