| Autor: |
James I. Matchynski, Rayyan Manwar, Karl J. Kratkiewicz, Rajtarun Madangopal, Veronica A. Lennon, Kassem M. Makki, Abbey L. Reppen, Alexander R. Woznicki, Bruce T. Hope, Shane A. Perrine, Alana C. Conti, Kamran Avanaki |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Photoacoustics, Vol 24, Iss , Pp 100297- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2213-5979 |
| DOI: |
10.1016/j.pacs.2021.100297 |
| Popis: |
Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large field-of-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an ex vivo imaging method in the Wistar Fos-LacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fos-expressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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