Co-Transfected SV40 Origin of Replication Activates Expression from SV40 Promoterless Constructs
| Autor: | A. Flint, S. Kaluz, M. Kaluzova, L. Sheldrick, P. Fisher, K. Derecka |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 27, Iss 4, Pp 728-732 (1999) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/99274st02 |
| Popis: | Co-transfection with expression plasmids is widely used to control DNA uptake efficiency in transient transfection experiments. However, a number of problems have been associated with their use. Here, we describe the activation of expression of constructs not containing the simian virus 40 (SV40) origin of replication (ori) by cotransfection in COS-7 cells with plasmids containing the SV40 ori. This effect has consequences for the use of such plasmids to control transfection efficiency. |
| Databáze: | Directory of Open Access Journals |
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