| Autor: |
Kouki Shiina, Masaya Komatsu, Fumi Yokoi, Hanako Bai, Masashi Takahashi, Manabu Kawahara |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
FASEB BioAdvances, Vol 1, Iss 7, Pp 393-403 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2573-9832 |
| DOI: |
10.1096/fba.2019-00005 |
| Popis: |
Abstract Oocyte spindle transfer (OST) is a potent reproductive technology used for mammals that enables the spindle in a deteriorated oocyte at the metaphase of the second meiotic division (MII) to serve as the genetic material for producing descendants. However, whether postnatal growth is achieved via OST using developmentally deteriorated MII oocytes remains unclear. At 16 h after human chorionic gonadotropin administration, denuded MII oocytes immediately after retrieval from oviducts (0 h‐oocytes) were used for in vitro fertilization (IVF) as controls. For IVF using postovulatory‐aged oocytes, the 0 h‐oocytes were further incubated for 12 h and 24 h (12 h‐ and 24 h‐oocytes). These mouse oocytes served as a model for assessing the postnatal growth of individuals produced via OST from developmentally deteriorated oocytes. The embryos from 12 h‐ and 24 h‐oocyte spindles exhibited high rates of development up to the neonatal stage as good as the non‐manipulated controls. However, the mice derived from the 24 h‐oocyte spindles displayed heavier body weights and greater feed consumption than both controls and mice derived from 12 h‐oocyte spindles. Our results demonstrate the feasibility of OST as a potent reproductive technology and its limitation in the use of excessively aged postovulatory oocytes in mammalian reproduction. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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