| Autor: |
T. Senthivelan, J. Kanagaraj, Rames C. Panda, T. Narayani |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Biotechnology Reports, Vol 23, Iss , Pp - (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2215-017X |
| DOI: |
10.1016/j.btre.2019.e00344 |
| Popis: |
This paper describes the isolation of potent extracellular-laccase producing white-rot fungus, identified by 18 s-rRNA as Penicillium-chrysogenum and its medium optimization by central-composite-rotatable-design using RSM. The optimum laccase-activity of 6.0 U ml-1 was obtained and maximum activity of 7.9 U ml-1 was achieved by statistical-optimization of the medium at 32 °C for 5 days. The molecular-weight of the laccase was found to be 67 kDa. UV-visible absorption-spectrum analysis shows peak at 600 nm and 325 nm corresponding to the type-I Cu(II) & type-III binuclear Cu(II) pair respectively confirming presence of laccase. The sharp endothermic peak at 150 °C and three-phases of protein denaturation was observed by DSC and TGA analysis for enzyme protein. The FT-IR analysis of laccase shows band at 1405cm-1, 1656 cm-1 &3400cm-1 corresponding to amide-I band, amide-II band and amino-acid group respectively. Results of the study show the enzyme is capable of carrying-out hydrolytic-cleavage of chemical-pollutants from the industrial waste-water for providing sustainable-greener environment Keywords: Laccase, Penicillium chrysogenum, RSM, redox potential, UV-vis spec, DSC and FT-IR |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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