Cell organelle-based analysis of cell chirality
| Autor: | Jie Fan, Haokang Zhang, Tasnif Rahman, Diana N. Stanton, Leo Q. Wan |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Communicative & Integrative Biology, Vol 12, Iss 1, Pp 78-81 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1942-0889 19420889 |
| DOI: | 10.1080/19420889.2019.1605277 |
| Popis: | The maintenance of tight endothelial junctions requires the establishment of proper cell polarity, which includes not only the apicobasal and front-rear polarity but also the left-right (L-R) polarity. The cell possesses an intrinsic mechanism of orienting the L-R axis with respect to the other axes, following a left-hand or right-hand rule, termed cell chirality. We have previously reported that endothelial cells exhibit a clockwise or rightward bias on ring-shaped micropatterns. Now we further characterize the chirality of individual endothelial cells on micropatterns by analyzing the L-R positioning of the cell centroid relative to the nucleus-centrosome axis. Our results show that the centroids of endothelial cells preferably polarized towards the right side of the nucleus-centrosome axis. This bias is consistent with cell chirality characterized by other methods. These results suggest that the positioning of cell organelles is intrinsically L-R biased inside individual cells. This L-R bias provides an opportunity for determining cell chirality in situ, even in vivo, without the limitations of using isolated cells in in vitro engineered platforms. |
| Databáze: | Directory of Open Access Journals |
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