Genetic diversity in a population of creole maize (Zea mays L.) evaluated by microsatellite markers in Puerto Libertador, Córdoba
| Autor: | Enrique Pardo Pérez, Teodora Cavadía Martínez, Yurany Herrera Vanegas |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Revista U.D.C.A Actualidad & Divulgación Científica, Vol 21, Iss 2 (2018) |
| Druh dokumentu: | article |
| ISSN: | 0123-4226 2619-2551 |
| DOI: | 10.31910/rudca.v21.n2.2018.981 |
| Popis: | Maize a plant of Mesoamerican origin, has evolved in different microenvironments, generating the great diversity of maize that exists in the world. In order to determine the genetic diversity of a population of Creole maize, twelve microsatellite markers were evaluated in 30 accessions, in Puerto Libertador, Córdoba. The DNA of each accession was extracted using the PROMEGA kit, the markers were amplified by the PCR technique and the amplicons were run on polyacrylamide gels, the gels were digitalized and the molecular sizes were determined by an exponential model. Results showed a total of 66 alleles and an average of alleles of 5.5, the expected heterozygosity was 0.655, the values of the polymorphic information content (PIC) ranged from 0.352 to 0.838, with an average of 0.592 and the Hardy-Weinberg equilibrium showed imbalance (p |
| Databáze: | Directory of Open Access Journals |
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