Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation

Autor:	Afsaneh Mojtabanezhad Shariatpanahi, Parisa Rokni, Elaheh Shahabi, Fatemeh Varshoee Tabrizi, Mohammad Amin Kerachian
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Plasma Liquid biopsy Cell-free DNA Medicine Biology (General) QH301-705.5 Science (General) Q1-390
Zdroj:	BMC Research Notes, Vol 11, Iss 1, Pp 1-6 (2018)
Druh dokumentu:	article
ISSN:	1756-0500
DOI:	10.1186/s13104-018-3866-8
Popis:	Abstract Objective In the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR. Results Using QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method.
Databáze:	Directory of Open Access Journals
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