Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform
| Autor: | Lukas Pekar, Michael Busch, Bernhard Valldorf, Steffen C. Hinz, Lars Toleikis, Simon Krah, Stefan Zielonka |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | mAbs, Vol 12, Iss 1 (2020) |
| Druh dokumentu: | article |
| ISSN: | 19420862 1942-0870 1942-0862 |
| DOI: | 10.1080/19420862.2020.1812210 |
| Popis: | Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody by two independently functioning VHH domains. Consequently, a VHH is engrafted onto constant region CH1 while the other VHH-based paratope is engrafted on the constant region of the light chain, Cκ or Cλ, resulting in a tetravalent bispecific IgG-like molecule. Combined with a heavy chain heterodimerization technique, this platform allows facile engineering of bi- and trispecific antibodies with flexible valencies. We demonstrate the general applicability of this generic platform approach and elaborate on the limitations of specific formats. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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