Transcriptome analysis of epithelial and stromal contributions to mammogenesis in three week prepartum cows.
| Autor: | Theresa Casey, Heather Dover, James Liesman, Lindsey DeVries, Matti Kiupel, Michael Vandehaar, Karen Plaut |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | PLoS ONE, Vol 6, Iss 7, p e22541 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1932-6203 44655916 |
| DOI: | 10.1371/journal.pone.0022541 |
| Popis: | Transcriptome analysis of bovine mammary development has provided insight into regulation of mammogenesis. However, previous studies primarily examined expression of epithelial and stromal tissues combined, and consequently did not account for tissue specific contribution to mammary development. Our objective was to identify differences in gene expression in epithelial and intralobular stromal compartments. Tissue was biopsied from non-lactating dairy cows 3 weeks prepartum, cut into explants and incubated for 2 hr with insulin and hydrocortisone. Epithelial and intralobular stromal tissues were isolated with laser capture microdissection. Global gene expression was measured with Bovine Affymetrix GeneChips, and data were preprocessed using RMA method. Moderated t-tests from gene-specific linear model analysis with cell type as a fixed effect showed more than 3,000 genes were differentially expressed between tissues (P |
| Databáze: | Directory of Open Access Journals |
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