| Autor: |
A. E. Efimov, O. I. Agapova, L. A. Safonova, M. M. Bobrova, V. A. Parfenov, E. V. Koudan, F. D. A. S. Pereira, E. A. Bulanova, V. A. Mironov, I. I. Agapov |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
eXPRESS Polymer Letters, Vol 13, Iss 7, Pp 632-641 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1788-618X |
| DOI: |
10.3144/expresspolymlett.2019.53 |
| Popis: |
We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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