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Abstract This study investigated the development, validation and applicability of a high-pressure liquid chromatographic (HPLC) method for analysis of eprinomectin (EPR) in thermoresponsive bioadhesive systems aiming the future pour-on administration in animals. For the determination of the both two types of EPR (B1a and B1b), an HPLC method was developed and validated according to the international standardized guidelines. A factorial design 22 plus central point was utilized for the system design, rendering five formulations containing poloxamer 407, carbomer 974P, isopropanol and EPR. The formulations were evaluated as visual characteristics and sol-gel transition temperature (Tsol/gel). In addition, the applicability of HPLC method was evaluated by analysis of the selected formulations, analyzing the drug content and trapping efficiency. The HPLC method was selective, linear, homoscedasticity, precise, accurate and robust. Formulations F3 and F5 presented instability; however, F1, F2 and F4 were considered suitable. Tsol/gel of F1, F2 and F4 were compatible for use on the animal skin, and they displayed good recovery for EPR content and trapping efficiency. The proposed HPLC method for eprinomectin analysis was valid and could determine the eprinomectin content in systems F1, F2 and F4. Moreover, the methodology showed to be of importance for EPR analysis in thermoresponsive systems, displaying applicability. |
