The ability of H2O2 to induce differentiation of human mesenchymal stem cells into chondrocytes
| Autor: | Goudarzi F, Mohammadalipour A, Bahabadi M, Moradi M.N, Sarveazad A, Goodarzi M.T, Khodadadi I |
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| Jazyk: | perština |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | مجله علمی دانشگاه علوم پزشکی کردستان, Vol 22, Iss 6, Pp 63-73 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1560-652X 2345-4040 |
| DOI: | 10.22102/22.6.63 |
| Popis: | Background and Aim:Cartilagedisordersmaydeterioratefollowingoxidative stress injuriesaffectingmature chondrocytes. Meantime, mesenchymal stem cells (MSCs)candifferentiateinto chondrocytes in thepresence ofoxidative conditionsandact as asourceofcompensationforinjured chondrocytes. The present study aimed to investigate the effect of H2O2on MSCsdifferentiation into chondrocytes in order tocastlight on the dual roles of oxidative stress inthepathogenesis of diseases.Materials and Methods:Human mesenchymal stem cells were isolated from abdominaladipose tissue of three different donors and cultured in the presence of 50MH2O2in orderto differentiate into chondrocytes.We determined cell viability by tetrazolium assayandmeasuredreactive oxygen species (ROS) level by flow cytometry.Presence ofglycoseaminoglycans was confirmed by safranin staining.Results:The percentage of cells containing ROS was significantly higher inthe cells treatedwithhydrogen peroxide (29.2%1) compared tothat in theuntreated control cells (7.7%1.4). A significant increase in glycoseaminoglycan content was observed in H2O2treatedcells comparedto that in thecontrol cells both onthe9thday (treated: 1.57×104± 0.1vscontrol: 0.91×104± 0.09) and21stday (treated: 2.87×104± 0.2vscontrol: 0.96×104± 0.07).In addition,comparison of glycoseaminoglycan content on the 9thand 21stdays showed asignificantly higher content inboth treated and control cells on the21stday(p |
| Databáze: | Directory of Open Access Journals |
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