| Autor: |
Wei Wu, Chuan Cheng, Zijiao Wang, Dongdong Yuan, Li Peng, Le Li |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 121, Iss , Pp 106458- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2024.106458 |
| Popis: |
Accumulating evidence suggests that M2-polarized tumor-associated macrophages (TAMs) play a crucial role in cancer progression, making them a promising target for therapy. This study explored the molecular mechanisms by which Platycodon grandiflorum regulates M2 polarization of TAMs to exert anti-tumor effects. UPLC-MS identified the chemical composition and blood-absorbed components. Immunohistochemistry and Western blot detected TAM infiltration and proliferation pathways, while q-RT-PCR measured M2 macrophage marker expression. Our findings showed that PR reduces melanoma weight, inhibits the mTOR pathway, and suppresses M2-polarized macrophages. PR downregulates JAK2 and p-STAT3, promotes JAK2 degradation, and enhances ubiquitination. Molecular docking indicated strong affinities between Platycodin D components and JAK2/STAT3, highlighting PR’s potential in regulating TAMs and offering new clinical insights. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect