| Autor: |
Jean-Philippe Mocho, Florian Lang, Guillaume Valentin, Sébastien Bedu, Robin McKimm, Juan Ramos, Yolanda Saavedra Torres, Sarah E. Wheatley, Joseph Higgins, Mollie E. Millington, Pia Rengtved Lundegaard, Rubén Chamorro Valverde, Vlasta Jenčič, Kristine von Krogh |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Biology, Vol 11, Iss 4, p 546 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2079-7737 |
| DOI: |
10.3390/biology11040546 |
| Popis: |
Euthanasia in zebrafish (Danio rerio) younger than 5 days post fertilization (dpf) is poorly described in the literature, and standardized protocols are lacking, most likely because larvae not capable of independent feeding are often not protected under national legislations. We assessed the euthanasia efficacy in laboratories in different countries of a one hour anesthetic overdose immersion with buffered lidocaine hydrochloride (1 g/L, with or without 50 mL/L of ethanol), buffered tricaine (1 g/L), clove oil (0.1%), benzocaine (1 g/L), or 2-phenoxyethanol (3 mL/L), as well as the efficacy of hypothermic shock (one hour immersion) and electrical stunning (for one minute), on zebrafish at |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
