Autor: |
Kirsten Rozemeijer, Fernando Dias Gonçalves Lima, Timo J. ter Braak, Albertus T. Hesselink, Jan M. Prins, Henry J.C. de Vries, Renske D.M. Steenbergen |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
Tumour Virus Research, Vol 17, Iss , Pp 200275- (2024) |
Druh dokumentu: |
article |
ISSN: |
2666-6790 |
DOI: |
10.1016/j.tvr.2023.200275 |
Popis: |
DNA methylation testing on biopsies can detect high-grade anal intraepithelial neoplasia (HGAIN) in need of treatment and anal cancer. This study aimed to analytically validate and determine the diagnostic performance of a newly developed multiplex quantitative methylation-specific PCR, PreCursor-M AnoGYN (RUO), combining ASCL1, ZNF582 and a reference (ACTB) in one assay. Analytical validation was performed on two qPCR devices using predefined quality criteria. Diagnostic performance was determined on a cross-sectional series of 111 anal biopsies covering all stages of anal disease. Differences in methylation levels were assessed using the Kruskal-Wallis test. Area under the curve was determined using logistic regression analysis. Detection rates were calculated at predefined specificities for the cross-sectional and an additional longitudinal series of 23 HGAIN biopsies preceding anal cancer (i.e., progressive HGAIN).For both devices analytical quality criteria were met. ASCL1 and ZNF582 methylation levels increased with increasing severity of disease (p |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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