Image Contrast Enhancement by Using LED Annular Oblique Illumination in Bright-Field Microscopy
| Autor: | Xiao Ma, Pan Qi, Zhifeng Wu, Ying Yang, Chengsen Li, Jingang Zhong |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Photonics, Vol 10, Iss 4, p 404 (2023) |
| Druh dokumentu: | article |
| ISSN: | 10040404 2304-6732 |
| DOI: | 10.3390/photonics10040404 |
| Popis: | Köhler illumination is the most used method for evenly illuminating a sample in bright-field microscopy. However, Köhler illumination cannot provide optimum contrast and high resolution simultaneously. The image contrast for Köhler illumination can be enhanced at the expense of lateral resolution. In this paper, we discuss the main causes of contrast loss with Köhler illumination by simulating unsymmetrical interference, incoherent superposition, and modulation transfer function. We present a method to increase the image contrast by combining the annular oblique illumination with normalization and blind deconvolution. With the progress of optoelectronic devices, the greater advantages of annular oblique illumination will become apparent. The experimental results achieved by using LED annular oblique illumination prove the feasibility of the proposed method. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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