| Autor: |
Javier E. Cañada-García, Zaira Moure, Pedro J. Sola-Campoy, Mercedes Delgado-Valverde, María E. Cano, Desirèe Gijón, Mónica González, Irene Gracia-Ahufinger, Nieves Larrosa, Xavier Mulet, Cristina Pitart, Alba Rivera, Germán Bou, Jorge Calvo, Rafael Cantón, Juan José González-López, Luis Martínez-Martínez, Ferran Navarro, Antonio Oliver, Zaira R. Palacios-Baena, Álvaro Pascual, Guillermo Ruiz-Carrascoso, Jordi Vila, Belén Aracil, María Pérez-Vázquez, Jesús Oteo-Iglesias, the GEMARA/GEIRAS-SEIMC/REIPI CARB-ES-19 Study Group, Mariela Martínez Ramírez, Pilar Zamarrón, Miriam Albert Hernández, M. Pilar Ortega Lafont, Emilia Cercenado, Cristobal del Rosario and Jose Luis Perez Arellano, María Lecuona, Luis López-Urrutia Lorente, José Leiva and José Luis del Pozo, Salvador Giner and Juan Frasquet, Lidia Garcia Agudo and Soledad Illescas, Pedro de la Iglesia, Rosario Sánchez Benito, Eugenio Garduño, Ma Isabel Fernández Natal and Marta Arias, Marta Lamata Subero, Mar Olga Pérez Moreno, Ana Isabel López-Calleja, Luis Torres Sopena, José Manuel Azcona, Alba Belles, Mercè García González, Miriam Valverde Troya and Begoña Palop, Fernando García Garrote, Jose Luis Barrios Andrés, Leyre López Soria, Adelina Gimeno, Susana Sabater, Ester Clapés Sanchez, Jennifer Villa, Nuria Iglesias Nuñez, Rafael Sánchez Arroyo, Inmaculada García García, Susana Hernando, Cristina Seral, Javier Castillo, Eva Riquelme Bravo, Caridad Sainz de Baranda, Oscar Esparcia Rodríguez, Jorge Gaitán, María Huertas, M.a José Rodríguez Escudero, Carmen Aldea, Nerea Sanchez, Antonio Casabella Pernas, Ma Dolores Quesada, Maria Pilar Chocarro, Francisco Javier Ramos, Carmina Martí Sala, Laura Mora, Encarnación Clavijo, Natalia Chueca, Federico García, José Gutierrez Fernández, Juan Manuel Sánchez Hospital de Jérez, Fátima Galán Sánchez, Carmen Liébana, Carolina Roldán, Ma Isabel Cabeza, José María Saavedra, Ma Teresa Cabezas Fernández, Lucía Martínez Lamas, Sonia Rey Cao, Ma Isabel Paz Vidal, Raquel Elisa Rodríguez Tarazona, Amparo Coira Nieto, Ma Luisa Pérez del Molino Bernal, María Gomáriz Díaz, Matxalen Vidal-García, Jose Luis Díaz de Tuesta, Moises García Bravo, Almudena Tinajas, Andrés Canut Blasco, Ma Luz Albina Cordón Rodriguez, Nieves Gonzalo Jiménez, Genoveva Yagüe Guirao, Fe Tubau Quintano, Carmen Aspiroz, Nuria Prim, Jesús Rodríguez-Baño |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology, Vol 13 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1664-302X |
| DOI: |
10.3389/fmicb.2022.918362 |
| Popis: |
ObjectivesCARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain.MethodsIn total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis.ResultsIn total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were blaOXA–48 (263/377), blaKPC–3 (62/377), blaVIM–1 (28/377), and blaNDM–1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5).ConclusionThis study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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