| Autor: |
Benoit-Vical Françoise, Nepveu Françoise, Parzy Daniel, Morlais Isabelle, Reybier Karine, Chevalley Séverine, Berry Antoine, Ribaut Clotilde, Valentin Alexis |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Malaria Journal, Vol 7, Iss 1, p 45 (2008) |
| Druh dokumentu: |
article |
| ISSN: |
1475-2875 |
| DOI: |
10.1186/1475-2875-7-45 |
| Popis: |
Abstract Background Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium. In this paper, an adaptation of magnetic MACS® columns for the purification of human Plasmodium species is presented. This method was useful for the concentration/purification of either schizonts or gametocytes. Results and conclusions The magnetic removal of non-parasitized red blood cells (in vivo and in vitro) using magnetic columns (MACS) was evaluated. This easy-to-use technique enriched schizonts and gametocytes from Plasmodium falciparum in vitro cultures with a very high degree of purity. In addition, all haemozoin-containing stages (schizonts and/or gametocytes) from the peripheral blood of infected patients could be concentrated using this method. This method is particularly useful for the concentration of non-falciparum species, which do not grow in culture and are otherwise difficult to obtain in large amounts. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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