Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

Autor: Lars H. Pedersen, Ole Bang, Poul E. Høiby, Grigoriy Emiliyanov
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Sensors, Vol 13, Iss 3, Pp 3242-3251 (2013)
Druh dokumentu: article
ISSN: 1424-8220
DOI: 10.3390/s130303242
Popis: We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.
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