| Autor: |
Guangwen Yan, Shuaibing Li, Yuhang Wen, Yadan Luo, Jingrong Huang, Baoting Chen, Shuya Lv, Lang Chen, Lvqin He, Manli He, Qian Yang, Zehui Yu, Wudian Xiao, Yong Tang, Weiyao Li, Jianhong Han, Fangfang Zhao, Shumin Yu, Fang Kong, Benazir Abbasi, Hongmei Yin, Congwei Gu |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Microbiology, Vol 13 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1664-302X |
| DOI: |
10.3389/fmicb.2022.1051200 |
| Popis: |
IntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.MethodsThe NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.ResultsCompared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.DiscussionTherefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
