| Autor: |
K. Walweel, A.C. Boon, L.E. See Hoe, N.G. Obonyo, S.E. Pedersen, S.D. Diab, M.R. Passmore, K. Hyslop, S.M. Colombo, N.J. Bartnikowski, M. Bouquet, M.A. Wells, D.M. Black, L.P. Pimenta, A.K. Stevenson, K. Bisht, K. Skeggs, L. Marshall, A. Prabhu, L.N. James, D.G. Platts, P.S. Macdonald, D.C. McGiffin, J.Y. Suen, J.F. Fraser |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Biomedical Journal, Vol 45, Iss 5, Pp 776-787 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2319-4170 |
| DOI: |
10.1016/j.bj.2021.10.007 |
| Popis: |
Introduction: Organs procured following brain stem death (BSD) are the main source of organ grafts for transplantation. However, BSD is associated with inflammatory responses that may damage the organ and affect both the quantity and quality of organs available for transplant. Therefore, we aimed to investigate plasma and bronchoalveolar lavage (BAL) pro-inflammatory cytokine profiles and cardiovascular physiology in a clinically relevant 6-h ovine model of BSD. Methods: Twelve healthy female sheep (37–42 Kg) were anaesthetized and mechanically ventilated prior to undergoing BSD induction and then monitored for 6 h. Plasma and BAL endothelin-1 and cytokines (IL-1β, 6, 8 and tumour necrosis factor alpha (TNF-α)) were assessed by ELISA. Differential white blood cell counts were performed. Cardiac function during BSD was also examined using echocardiography, and cardiac biomarkers (A-type natriuretic peptide and troponin I were measured in plasma. Results: Plasma concentrations big ET-1, IL-6, IL-8, TNF-α and BAL IL-8 were significantly (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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