Ectopic expression of the Osmyb4 rice gene enhances synthesis of hydroxycinnamic acid derivatives in tobacco and clary sage

Autor:	T. Docimo, M. Mattana, R. Fasano, R. Consonni, N. de Tommasi, I. Coraggio, A. Leone
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	chlorogenic acid myb transcription factor nicotiana tabacum phenylpropanoid pathway rosmarinic acid salvia sclarea Biology (General) QH301-705.5 Plant ecology QK900-989
Zdroj:	Biologia Plantarum, Vol 57, Iss 1, Pp 179-183 (2013)
Druh dokumentu:	article
ISSN:	1573-8264
DOI:	10.1007/s10535-012-0257-1
Popis:	In this work, we report the ectopic expression of the Osmyb4 rice gene, encoding the Myb4 transcription factor, in Nicotiana tabacum and Salvia sclarea. Transcriptional analysis of T2 homozygous tobacco plants overexpressing Osmyb4 revealed that Myb4 activated the transcription of several genes of the phenylpropanoid pathway such as PAL, C4H, 4CL1, 4CL2 (encoding phenylalanine ammonia-lyase, cinnamic acid 4-hydroxylase, 4-coumarate: Co A ligase1, 4-coumarate: Co A ligase2). Moreover, the Myb4 increased expression of HQT encoding hydroxycinnamoyl-CoA: quinate transferase, which specifically triggers the accumulation of chlorogenic acid (CGA). In addition, increased acccumulation of rosmarinic acid (RA) was found in transgenic plants of both species. These results open the possibility of using the Osmyb4 gene to increase the production of specific bioactive hydroxycinnamates.
Databáze:	Directory of Open Access Journals
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