A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
| Autor: | George Jîtcă, Erzsébet Fogarasi, Bianca-Eugenia Ősz, Camil Eugen Vari, Amelia Tero-Vescan, Amalia Miklos, Mădălina-Georgiana Bătrînu, Carmen Maria Rusz, Mircea Dumitru Croitoru, Maria Titica Dogaru |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Molecules, Vol 26, Iss 16, p 5066 (2021) |
| Druh dokumentu: | article |
| ISSN: | 26165066 1420-3049 |
| DOI: | 10.3390/molecules26165066 |
| Popis: | In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|