Synergistic Effect of Hyperbaric Oxygen and Decellularized Human Amniotic Membrane on Full-Thickness Diabetic Wound Healing in Rats
| Autor: | Mostafa Izanlu, Alireza Khalatbary, Amin Aliabadi, Sima Davoodi, Amir Raoofi, Faezeh Modanloo, Davood Nasiry |
|---|---|
| Jazyk: | English<br />Persian |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Journal of Mazandaran University of Medical Sciences, Vol 32, Iss 208, Pp 1-15 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1735-9260 1735-9279 |
| Popis: | Background and purpose: One of the common complications of diabetes is impairment of wound healing. The aim of the present study was to investigate the synergistic effect of hyperbaric oxygen (HBO) therapy and decellularized human amniotic membrane (dHAM) in healing of full-thickness diabetic wounds in rats. Materials and methods: In this experimental study, 60 diabetic male rats were randomly divided into four groups (n=15) as follows: untreated diabetic wound, wound treated with HBO, wound treated with dHAM, and wound treated with HBO+dHAM. Sampling was performed after 7, 14, and 21 days following wounding. Assessments included wound closure rate, histological (collagen density) and stereological examinations, expression of genes involved in repair and angiogenesis (TGF-β and VEGF), inflammatory genes (TNF-α and IL-1β), and wound tissue biomechanical properties. Results: Combined use of dHAM and HBO significantly increased wound closure rate, new epidermis and dermis volume, fibroblast cell and blood vessel density, collagen density, repair and angiogenesis genes, and wound biomechanical properties compared with those in the diabetic group (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|