| Autor: |
Prajwal C. Boddu, Abhishek K. Gupta, Jung-Sik Kim, Karla M. Neugebauer, Todd Waldman, Manoj M. Pillai |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Communications Biology, Vol 4, Iss 1, Pp 1-9 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2399-3642 |
| DOI: |
10.1038/s42003-021-02690-1 |
| Popis: |
Boddu et al combined AAV-intron trap, CRISPR/Cas9 and inducible Cre-recombinase systems to introduce the oncogenic K700E mutation in SF3B1, which is a splicing factor commonly mutated in multiple cancers, with more than 90% efficiency. Their approach can be readily adapted to generate scalable isogenic systems where mutant oncogenes are detrimental to cell culture growth. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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