Effect of fermentation time on phenolic content and antioxidant potential in Cupuassu (Theobroma grandiflorum (Willd. ex Spreng.) K.Schum.) beans

Autor: Laura Cuellar Alvarez, Natalia Cuellar Alvarez, Paula Galeano Garcia, Juan Carlos Suárez Salazar
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2017
Předmět:
Zdroj: Acta Agronómica, Vol 66, Iss 4, Pp 473-479 (2017)
Druh dokumentu: article
ISSN: 0120-2812
2323-0118
19714149
DOI: 10.15446/acag.v66n4.61821
Popis: Cupuassu (Theobroma grandiflorum (Willd. ex Spreng.) K.Schum.) is an evergreen tree in the family Malvaceae, with nutritional qualities of interest in the food and cosmetic industry. It is necessary for its processing, in addition to other processes, to perform a fermentation, affecting its chemical composition. Therefore, the effect of fermentation time on the phenolic content and antioxidant activity of Cupuassu (T. grandiflorum) beans, was determined. During this process, the chemical properties of the beans and the phenolic content were evaluated every two days; also quantifying the secondary metabolites Catechin, Epicatechin, Theobromine and Caffeine by high performance liquid chromatography (HPLC). The antioxidant activity was analyzed using the ABTS, DPPH, and FRAP assays. Analysis of phenolic content and antioxidant activity showed a decrease after 6 days of fermentation. Therefore, it is not recommended to continue fermentation after this period due to a negative influence of the process on the bioactive substances (Catechins) content, and the reduction of the ability to inhibit free radicals, exhibited by Cupuassu beans.
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