Cloning of adult cows by nuclear transfer of ovarian mural granulosa cells
| Autor: | I. Lagutina, D. Ponce, R. Diaz, F. Castañeda, I. Mesía |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | SPERMOVA, Vol 9, Iss 1, Pp 19-27 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2223-9375 2308-4928 |
| DOI: | 10.18548/aspe/0007.03 |
| Popis: | The objective was cloning three adult cows using their ovarian mural granulosa cells collected by transvaginal ovum pick up (OPU). One group (T1) of granulosa cells were matured together with their oocytes as part of the COC (Cumulus Oocyte Complex), a second group (T2) were cultured for 3 days independently of their oocytes and a third (T3) group was collected from the cells that are shed during the filtration of the OPU aspirations. The oocytes were denuded and then enucleated after previous treatment with pronase to digest the zona pellucida. The reconstructs were subjected to electro fusion before the chemical activation with ionomycin. A total of 324 embryo reconstructs were obtained; 158 from T1, 45 from T2 and 121 from T3. The overall response to activation of the embryo reconstructs was 91.35% and there were no statistical differences (P>0.05) between treatments. There were however significant differences (p |
| Databáze: | Directory of Open Access Journals |
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