Central Carbon Metabolism in Candida albicans Biofilms Is Altered by Dimethyl Sulfoxide

Autor: Maria Fernanda Cordeiro Arruda, Romeu Cassiano Pucci da Silva Ramos, Nicoly Subtil de Oliveira, Rosimeire Takaki Rosa, Patrícia Maria Stuelp-Campelo, Luiz Fernando Bianchini, Silas Granato Villas-Bôas, Edvaldo Antonio Ribeiro Rosa
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Fungi, Vol 10, Iss 5, p 337 (2024)
Druh dokumentu: article
ISSN: 10050337
2309-608X
DOI: 10.3390/jof10050337
Popis: The effect of dimethyl sulfoxide (DMSO) on fungal metabolism has not been well studied. This study aimed to evaluate, by metabolomics, the impact of DMSO on the central carbon metabolism of Candida albicans. Biofilms of C. albicans SC5314 were grown on paper discs, using minimum mineral (MM) medium, in a dynamic continuous flow system. The two experimental conditions were control and 0.03% DMSO (v/v). After 72 h of incubation (37 °C), the biofilms were collected and the metabolites were extracted. The extracted metabolites were subjected to gas chromatography–mass spectrometry (GC/MS). The experiment was conducted using five replicates on three independent occasions. The GC/MS analysis identified 88 compounds. Among the 88 compounds, the levels of 27 compounds were markedly different between the two groups. The DMSO group exhibited enhanced levels of putrescine and glutathione and decreased levels of methionine and lysine. Additionally, the DMSO group exhibited alterations in 13 metabolic pathways involved in primary and secondary cellular metabolism. Among the 13 altered pathways, seven were downregulated and six were upregulated in the DMSO group. These results indicated a differential intracellular metabolic profile between the untreated and DMSO-treated biofilms. Hence, DMSO was demonstrated to affect the metabolic pathways of C. albicans. These results suggest that DMSO may influence the results of laboratory tests when it is used as a solvent. Hence, the use of DMSO as a solvent must be carefully considered in drug research, as the effect of the researched drugs may not be reliably translated into clinical practice.
Databáze: Directory of Open Access Journals
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