| Autor: |
Vu Thi Ngoc Bich, Le Viet Thanh, Pham Duy Thai, Tran Thi Van Phuong, Melissa Oomen, Christel Driessen, Erik Beuken, Tran Huy Hoang, H. Rogier van Doorn, John Penders, Heiman F. L. Wertheim |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Antimicrobial Resistance and Infection Control, Vol 8, Iss 1, Pp 1-10 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2047-2994 |
| DOI: |
10.1186/s13756-019-0645-9 |
| Popis: |
Abstract Background Antibiotic resistance is a major global public health threat. Antibiotic use can directly impact the antibiotic resistant genes (ARGs) profile of the human intestinal microbiome and consequently the environment through shedding. Methods We determined the resistome of human feces, animal stools, human food and environmental (rain, well, and irrigative water) samples (n = 304) in 40 households within a community cohort and related the data to antibiotic consumption. Metagenomic DNA was isolated and qPCR was used to determine presence of mobile colistin resistance (mcr) genes, genes encoding extended-spectrum β-lactamases (ESBL), carbapenemases and quinolone resistance genes. Results Nearly 40 % (39.5%, 120/304) of samples contained ESBL genes (most frequent were CTX-M-9 (23.7% [72/304]), CTX-M-1 (18.8% [57/304]). Quinolone resistance genes (qnrS) were detected in all human and 91% (41/45) of animal stool samples. Mcr-1 and mcr-3 were predominantly detected in human feces at 88% (82/93) and 55% (51/93) and animal feces at 93% (42/45) and 51% (23/45), respectively. Mcr-2, mrc-4 and mcr-5 were not detected in human feces, and only sporadically ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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