| Autor: |
Elżbieta Kuźniak, Marzena Wielanek, Urszula Małolepsza, Henryk Urbaniak |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Acta Agrobotanica, Vol 48, Iss 2, Pp 113-120 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
2300-357X |
| DOI: |
10.5586/aa.1995.022 |
| Popis: |
The effects of soil or in vitro grown plants, pretreatment conditions, donor tissue and isolation procedure on protoplast yield from cotyledons and leaves of tomato cv. 'Perkoz' and 'Zorza' were studied. The highest protoplast yield of 1.5 x 107/g FW was obtained from leaves of in vitro grown plants. Low light intensity during donor plants in vitro culture and dark pretreatment were essential for successful protoplast isolation while cold pretreatment was not. Tissue preplasmolysis prior to transfer to enzyme mixture increased 4-fold the number of isolated protoplasts. Glycine and bovine serum albumin in the isolation medium did not significantly influence the protoplast yield. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
