Potential of RH5 Antisense on Plasmodium falciparum Proliferation Abatement
| Autor: | Sepand Razavi Vakhshourpour, Mehdi Nateghpour, Nader Shahrokhi, Afsaneh Motevalli Haghi, Mehdi Mohebali, Haleh Hanifian |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Iranian Journal of Parasitology, Vol 17, Iss 4 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1735-7020 2008-238X |
| DOI: | 10.18502/ijpa.v17i4.11280 |
| Popis: | Background: Infections by Plasmodium falciparum, are becoming increasingly difficult to treat. Therefore, there is an urgent need for novel antimalarial agents’ discovery against infection. In present study, we described a 2’-O-Methyl gapmer phosphorothioate oligonucleotide antisense targeting translation initiation region of 3D7 strain RH5 gene. Methods: The study was conducted in Pasteur Institute of Iran in 2020. ODNs effects were measured by microscopic examination and real time RT-PCR. For microscopy, microplates were charged with 2’-OMe ODNs at different dilutions. Unsynchronized parasites were added to a total of 0.4 ml (0.4% parasitemia, 5% red blood cells), and slides were prepared. Proportion of infected cells was measured by counting at least 500 red blood cells. Results: RH5 genes start codon regions selected as conserved region besed on alignment results. Gap-RH5-As which was complementary to sequence surrounding AUG RH5 start codon significantly reduced parasite growth (>90% at 50 nM) compared to sense sequence control (Gap-RH5-Se) (17%), (P |
| Databáze: | Directory of Open Access Journals |
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