Effects of aroma quality on the biotransformation of natural 2-phenylethanol produced using ascorbic acid

Autor: Xun Tian, Rui Ye, Jingwen Wang, Yifei Chen, Baoguo Cai, Shimin Guan, Shaofeng Rong, Qianqian Li
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Electronic Journal of Biotechnology, Vol 18, Iss 4, Pp 286-290 (2015)
Druh dokumentu: article
ISSN: 0717-3458
DOI: 10.1016/j.ejbt.2015.05.003
Popis: Background: Natural 2-phenylethanol (2-PE) is an important flavoring that emits the aroma of roses. During biotransformation, the aroma quality of natural 2-PE is affected by its main by-products, which include butanol, isobutyric acid, butyric acid, and isovaleric acid. Thus, controlling undesirable by-product formation can reduce the effect of odor on 2-PE aroma quality. Results: 2-PE was produced through biotransformation using l-phenylalanine as a substrate and glucose as a carbon source. Ascorbic acid was added to the system to improve the redox reaction and suppress the generation of by-products. Principal component analysis of the aroma quality of 2-PE was performed using an electronic nose. Similarity analysis revealed that the effects of four by-products on 2-PE aroma quality may be ranked in the following order: isovaleric acid > butyric acid > isobutyric acid > butanol. The sample that exhibited the best similarity to the standard 2-PE sample (99.19%) was the sample to which ascorbic acid had been added during glucose metabolism. Conclusions: 2-PE produced through the addition of ascorbic acid exhibited the closest aroma similarity to the standard 2-PE sample.
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