The sigma-and omega-class members of the glutathione-S-transferase family from ascaris are IgE binding components with marked differences in the IgG1 and IgG4 response

Autor: Ana Milena Lozano-Mendoza, Juana Bustillo, Juan López, Luis Caraballo, Josefina Zakzuk
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2018
Předmět:
Zdroj: Revista Alergia México, Vol 65 suppl 1, Pp 145-146 (2018)
Druh dokumentu: article
ISSN: 0002-5151
2448-9190
Popis: Background: The family of gluthation-S-transferases possesses several isoforms, one of its members, Asc s 13, is allergenic, but other molecules of this repertoire may have different immunological properties. Different omega-class of gluthatio-S-transferases has been reported as anti-inflammatory molecules. Objective: To evaluate the antibody response to a new member of the gluthation-S-transferases family from Ascaris suum. Methods: Recombinant gluthatio-S-transferases omega (rGSTO) and gluthatio-S-transferases sigma or Asc s 13 (rGSTA) from Ascaris suum were obtained in Escherichia coli. Specific IgE, IgG4 and IgG1 levels were determined by ELISA in 183 patients from a rural area of Colombia where ascariasis is endemic. A phylogenetic tree was built with published helminth gluthatio-S-transferases sequences. IgG reactivity to rGSTO and rGSTA was evaluated by ELISA using a polyclonal antibody (pAb) obtained from rGSTA-immunized mice. Results: These proteins share 20 %/39 % of identity/similarity; low surface amino-acid conservation was also observed. These Ascaris suum-gluthatio-S-transferases clustered in different clades, rGSTO was more similar to other omega-gluthatio-S-transferases and rGSTA to other sigma-class. Anti-rGSTA pAb recognized the immunogen in a dilution-dependent manner, but not the rGSTO. Human IgE recognized both, rGSTA (median OD 405 = 0.127; IQR = 0.111-0.150) and rGSTO (0.122; IQR = 0.109-0.157), with no difference (p = 0.321). Sensitization rates were similar (rGSTA: 32.8 % and rGSTO: 30.6 %, p = 0.65). Human-IgG4 against rGSTO and rGSTA was found in 73.2 % and 41.0 % (p
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