Identifying Mycobacterium species and strain typing using a microfluidic labchip instrument
| Autor: | Robert C. Cooksey, Josef Limor, Glenn P. Morlock, Jack T. Crawford |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 35, Iss 4, Pp 786-794 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/03354rr01 |
| Popis: | We developed schemes for rapid identification of Mycobacterium species and strain typing using a microfluidic labchip instrument. A 439-bp region of the gene that codes for the 65-kDa heat shock protein (hsp65), which has sequence polymorphisms specific for most mycobacterial species, was examined using PCR-restriction analysis (PRA). We performed PRA in duplicate, using 2 strains each of 12 species, and observed that fragment sizes (bp) determined automatically by the instrument were consistently smaller than the correct sizes for each of the species as determined by sequence analysis (mean variance, |
| Databáze: | Directory of Open Access Journals |
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