Effect of pFSH dose reduction on in vivo embryo production in Dorper ewes

Autor: João Bosco Loiola Filho, Alane Pains Oliveira do Monte, Thais Thatiane dos Santos Souza, Mayara de Souza Miranda, Lívia Correia Magalhães, Celso Henrique Souza Costa Barros, Ana Arlete de Amorim Silva, Adriano Oliveira Santos, Aionne de Souza Leite Guimarães, Jonathan Maia da Silva Costa, Rodrigo Bonfim Cruz, Mabel Freitas Cordeiro, Edilson Soares Lopes Júnior
Jazyk: English<br />Portuguese
Rok vydání: 2015
Předmět:
Zdroj: Semina: Ciências Agrárias, Vol 36, Iss 6Supl2, Pp 4215-4224 (2015)
Druh dokumentu: article
ISSN: 1676-546X
1679-0359
DOI: 10.5433/1679-0359.2015v36n6Sup2p4215
Popis: To evaluate the effect of pFSH dose on the in vivo embryo production of Dorper ewes in the semi-arid northeast of Brazil, 40 sheep females were distributed into two groups of 20 animals that received intravaginal CIDR for 14 days, and two days before device removal, they received one of the following treatments: in the FSH200 group, the ewes received 200 mg of pFSH; and in the FSH128 group, the ewes received a total of 128 mg in decreasing doses every 12 h. Beginning 12 h after the conclusion of the treatments, estrus detection was performed every four hours using two Dorper rams of proven fertility. The ewes were mated at estrus onset and 24 hours later. Seven days after intravaginal device removal, the superovulatory response was evaluated, and embryo collection was performed using the laparotomy method. The recovered flushings were subjected to embryo searches under a stereomicroscope and classified according to their qualities. Analyses of variance (ANOVAs) and LSD tests were used to compare the different parameters. The data expressed as percentages were analysed by chi-square test. The ovulation rate was higher in the FSH200 group, which had 16.3 ± 0.3 corpora lutea (CL), than in the FSH128 group, which had 11.3 ± 0.3 CL (P0.05), demonstrating that pFSH dose reduction promoted a greater production of freezable and transferable embryos in Dorper ewes subjected to MOET.
Databáze: Directory of Open Access Journals
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