Effect of low concentration of sodium fluoride on osteogenic/odontogenic differentiation of human dental pulp cells
| Autor: | LI Lifen, HAN Junli, JIANG Long |
|---|---|
| Jazyk: | čínština |
| Rok vydání: | 2024 |
| Předmět: |
human dental pulp cell
sodium fluoride proliferation osteogenic/odontogenic differentiation endoplasmic reticulum stress splicing xbox binding protein 1 activating transcription factor 4 glucose-regulated protein 78 rna-activated protein kinase-like er-resident kinase eukaryotic initiation factor-2α Medicine |
| Zdroj: | 口腔疾病防治, Vol 32, Iss 1, Pp 22-28 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2096-1456 93141483 |
| DOI: | 10.12016/j.issn.2096-1456.2024.01.004 |
| Popis: | Objective To study the effect of low concentrations of sodium fluoride on the osteogenic/odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. Methods This study was reviewed and approved by the Ethics Committee. hDPCs were cultured using a modified tissue explant technique in vitro. The effects of different concentrations of sodium fluoride on the proliferation of hDPCs were measured by methylthiazol tetrazolium (MTT) assay. Appropriate concentrations were added to the osteogenic/odontogenic differentiation induction medium, and the cells were induced in vitro. Alizarin red S staining was used to detect the osteoblastic/odontogenic differentiation ability of the cells, and the mRNA expression of the key differentiation factors was detected by RT-qPCR. Moreover, the expression of key molecules of endoplasmic reticulum stress (ERS) was detected by RT-qPCR and Western blot. The data were analyzed with the SPSS 18.0 software package. Results Low concentration of NaF (0.1 mmol/L) could stimulate cell proliferation in vitro, while a high concentration (5-10 mmol/L) could inhibit cell proliferation (P |
| Databáze: | Directory of Open Access Journals |
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