Effects of N-Terminus Substitution on the Structure and Spectroscopy of Gas-Phase Helices
| Autor: | Jaime A. Stearns, Oleg V. Boyarkin, Thomas R. Rizzo |
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| Jazyk: | German<br />English<br />French |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | CHIMIA, Vol 62, Iss 4 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0009-4293 2673-2424 |
| DOI: | 10.2533/chimia.2008.240 |
| Popis: | Because of the importance of helices in the secondary structure of proteins, we have undertaken a study of their spectroscopy, fragmentation patterns and conformations in the gas phase. In this work, we describe the effects of substitution at the N-terminus of polyalanine helices capped with a lysine at the C-terminus, namely Ac-Phe-(Ala)10-Lys-H+, Phe-(Ala)10-Lys-H+, and H+-Phe-(Ala)10-Lys-H+. Acetylation of the N-terminus has very little effect on the spectroscopy and structure, but protonation of the N-terminus changes the infrared spectrum in such a way that we believe it may also change the structure of the peptide. The ultraviolet spectroscopy and fragmentation, on the other hand, are insensitive to either acetylation or protonation. |
| Databáze: | Directory of Open Access Journals |
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