A Single Complementary-Sense Transcript of a Geminiviral DNA β Satellite Is Determinant of Pathogenicity
| Autor: | Muhammad Saeed, S. A. Akbar Behjatnia, Shahid Mansoor, Yusuf Zafar, Shahida Hasnain, M. Ali Rezaian |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Molecular Plant-Microbe Interactions, Vol 18, Iss 1, Pp 7-14 (2005) |
| Druh dokumentu: | article |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/MPMI-18-0007 |
| Popis: | Small circular single-stranded DNA satellites, termed DNAβ, have recently been found associated with some geminivirus infections. The DNA β associated with Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan. Mutagenesis of DNA β revealed that the complementary-sense open reading frame (ORF) βC1 is required for inducing disease symptoms in Nicotiana tabacum. An ORF present on the virion-sense strand βV1 appeared to have no role in pathogenesis. Tobacco plants transformed with a βC1 ORF under the control of the Cauliflower mosaic virus 35S promoter or with a dimeric DNA β exhibited severe disease-like phenotypes, while plants transformed with a mutated version of βC1 appeared normal. Northern blot analysis of RNA from the transgenic plants, using strand-specific probes, identified a single complementary-sense transcript. The transcript carries the full βC1 ORF encoding a 118-amino acid product. It maps to the DNA β at nucleotide position 186 to 563 and contains a polyadenylation signal 18 nt upstream of the stop codon. A TATA box is located 43 nt upstream of the start codon. Our results indicate that βC1 protein is responsible for DNA β-induced disease symptoms. |
| Databáze: | Directory of Open Access Journals |
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