Circ_0000317/microRNA‐520g/HOXD10 axis affects the biological characteristics of colorectal cancer

Autor: Fu‐Ji Lai, Hua Yu, Yang‐Yang Xie, Ning He
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Kaohsiung Journal of Medical Sciences, Vol 37, Iss 11, Pp 951-963 (2021)
Druh dokumentu: article
ISSN: 2410-8650
1607-551X
86790145
DOI: 10.1002/kjm2.12422
Popis: Abstract Circular RNAs (circRNAs) are a class of noncoding RNAs that are widely expressed in cancer tissues and play a pro‐ or anticancer role in modulating cancer progression. This work is aimed to probe the biological role of circ_0000317 in colorectal cancer (CRC) and its underlying mechanism. Circ_0000317 was selected from the circRNA microarray datasets (GSE121895). Quantitative real‐time polymerase chain reaction was utilized to examine circ_0000317, microRNA (miR)‐520g, and homeobox D10 (HOXD10) mRNA expression in CRC. Cell Counting Kit‐8 and Transwell experiments were conducted to examine the effects of circ_0000317 on proliferation, migration, and invasion of CRC cells. Bioinformatic analysis and dual‐luciferase reporter gene experiments were implemented to predict and validate the targeting relationship between circ_0000317 and miR‐520g, miR‐520g, and HOXD10. Western blot was employed to examine HOXD10 expression at protein level in CRC cells. Circ_0000317 and HOXD10 mRNA expression were unveiled to be down‐modulated and miR‐520g expression was up‐modulated in CRC. Functionally, circ_0000317 overexpression repressed CRC cell proliferation, migration, and invasion. Mechanistically, miR‐520g was a direct target of circ_0000317 and miR‐520g specifically modulated HOXD10 expression. Furthermore, miR‐520g mimics partially counteracted the suppressing effect of circ_0000317 on malignant phenotype of CRC cells. Circ_0000317 represses CRC progression by targeting miR‐520g and modulating HOXD10 expression. Hence, circ_0000317 may be a promising diagnostic biomarker and a therapeutic target for CRC.
Databáze: Directory of Open Access Journals
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