| Autor: |
Ali Zarghami Khameneh, Saeed Nikookheslat, Afshar Jafari, Pouran Karimi |
| Jazyk: |
perština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Complementary Medicine Journal of Faculty of Nursing and Midwifery, Vol 10, Iss 3, Pp 206-217 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2228-7094 |
| DOI: |
10.32598/cmja.10.3.460.6 |
| Popis: |
Objective: Some previous studies have shown the protective effect of caffeine on apoptosis through the regulation of pro- and anti-apoptotic proteins. The aim of this study was to investigate the effects of chronic caffeine administration on the expression ratio of B-cell lymphoma protein 2 (Bcl-2) and Bcl-2-Associated X-protein (Bax) proteins in the cardiac tissue of rats with Type 2 Diabetes. Methods: In this experimental study, samples were 24 male white wistar rats (aged 2-3 months with a weight of 250-300 g) randomly divided into three groups: Healthy control (n=8), untreated diabetic (n=8), and diabetic with caffeine supplement (n=8; 70 mg/kg-1 for 8 weeks, 5 days a week). The expression of proteins associated with apoptotic signaling pathway (Bax and Bcl-2) in the cardiac muscle (left ventricular) was measured by Western blot method. One-Way Variance (ANOVA), t-test, and Tukey’s post hoc test were used for data analysis. Results: Induction of type two diabetes significantly increased the expression of Bax protein (1.81±0.2) and decreased the expression of Bcl-2 protein (0.36±0.05) compared to control group (P=0.001). However, caffeine administration increased the expression of Bax protein (131%) compared to diabetic control group (P=0.001). Therefore, caffeine administration after diabetes induction elevated the Bax/Bcl-2 ratio (P=0.001). Conclusion: Eight weeks of caffeine administration have an exacerbating effect on the apoptotic cell death caused by type 2 diabetes by increasing pro-apoptotic proteins and reducing anti-apoptotic proteins. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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