| Autor: |
LI Jiapeng, XIE Qiurong, WEN Ying, CHEN Haichun, PENG Jun, SHEN Aling |
| Jazyk: |
English<br />Chinese |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
康复学报, Vol 32, Pp 232-242 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2096-0328 |
| DOI: |
10.3724/SP.J.1329.2022.03006 |
| Popis: |
ObjectiveTo investigate the effects of swimming on the growth of colorectal cancer (CRC) transplanted tumor in mice, and to explore its regulation mechanism from the perspective of gut microbiota and inflammation.MethodsThe CT-26 bearing mouse model was constructed and the experiment mice were randomly divided into model and swimming groups. The mice in the swimming group were subjected to physical training of swimming for 30 minutes each time and twice per day, 6 days per week for 3 weeks in total. The fresh feces of all mice at 24 hours after the last swimming were collected and stored in liquid nitrogen. The collected tumor tissues were dissected and weighted. The compositions of gut microbiota were analyzed by 16S rRNA sequencing. The functional enrichment of gut microbiota in mice was evaluated by KEGG to compare the differences of metabolic pathways of functional genes in the intestinal flora between the two groups. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of TNF-α, IL-1β and IL-10 in serum of mice. SPSS 26.0 and GraphPad Prism 7 software were used for statistical analysis.ResultsCompared with the model group, swimming significantly reduced the weight of CRC transplanted tumor in mice (PPPPPPP |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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