Molecular cloning, sequence identification and expression profile of domestic guinea pig (Cavia porcellus) UGT1A1 gene
| Autor: | Yang Deming, An Qingcong, Liu Yonggang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Biotechnology & Biotechnological Equipment, Vol 30, Iss 1, Pp 106-111 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1310-2818 1314-3530 13102818 |
| DOI: | 10.1080/13102818.2015.1098565 |
| Popis: | Domestic guinea pig is a model animal for human disease research. Uridine diphosphate glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1) is an important human disease-related gene. In this study, the complete coding sequence of domestic guinea pig gene UGT1A1 was amplified by reverse transcription-polymerase chain reaction. The open reading frame of the domestic guinea pig UGT1A1 gene is 1602 bp in length and was found to encode a protein of 533 amino acids. Sequence analysis revealed that the UGT1A1 protein of domestic guinea pig shared high homology with the UGT1A1 proteins of degu (84%), damara mole-rat (84%), human (80%), northern white-cheeked gibbon (80%), Colobus angolensis palliatus (80%) and golden snub-nosed monkey (79%). This gene contains five exons and four introns, as revealed by the computer-assisted analysis. The results also showed that the domestic guinea pig UGT1A1 gene had a close genetic relationship with the UGT1A1 gene of degu. The prediction of transmembrane helices showed that domestic guinea pig UGT1A1 might be a transmembrane protein. Expression profile analysis indicated that the domestic guinea pig UGT1A1 gene was differentially expressed in detected domestic guinea pig tissues. Our experiment laid a primary foundation for using the domestic guinea pig as a model animal to study the UGT1A1-related human diseases. |
| Databáze: | Directory of Open Access Journals |
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