Complete Nucleotide Sequence, Genome Organization, and Comparative Genomic Analyses of Citrus Yellow-Vein Associated Virus (CYVaV)

Autor: Sun-Jung Kwon, Sohrab Bodaghi, Tyler Dang, Kiran R. Gadhave, Thien Ho, Fatima Osman, Maher Al Rwahnih, Ioannis E. Tzanetakis, Anne E. Simon, Georgios Vidalakis
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Frontiers in Microbiology, Vol 12 (2021)
Druh dokumentu: article
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.683130
Popis: Citrus yellow-vein disease (CYVD) was first reported in California in 1957. We now report that CYVD is associated with a virus-like agent, provisionally named citrus yellow-vein associated virus (CYVaV). The CYVaV RNA genome has 2,692 nucleotides and codes for two discernable open reading frames (ORFs). ORF1 encodes a protein of 190 amino acid (aa) whereas ORF2 is presumably generated by a −1 ribosomal frameshifting event just upstream of the ORF1 termination signal. The frameshift product (717 aa) encodes the RNA-dependent RNA polymerase (RdRp). Phylogenetic analyses suggest that CYVaV is closely related to unclassified virus-like RNAs in the family Tombusviridae. Bio-indexing and RNA-seq experiments indicate that CYVaV can induce yellow vein symptoms independently of known citrus viruses or viroids.
Databáze: Directory of Open Access Journals